Myocardial mitochondria are primary sites of myocardial energy metabolism. Mitochondrial disorders are associated with various cardiac diseases. We previously showed that mice with cardiomyocyte-specific knockout of the mitochondrial translation factor p32 …
Most protein-coding genes in higher eukaryotes are subject to alternative processing yielding multiple mRNA isoforms, thereby diversifying the functional transcriptome (Tian & Manley, 2017; Ule & Blencowe, 2019). The ability to generate complex …
Introduction In humans, heterozygous point mutations in FAM111A are linked to two severe developmental syndromes: the Kenny–Caffey syndrome (KCS2, OMIM-127000) and Gracile bone dysplasia (GCLEB, OMIM-602361). In both diseases, patients are characterized by …
Anchorage-independent survival after intravasation of cancer cells from the primary tumor site represents a critical step in metastasis. Here, we reveal new insights into how MUC13-mediated anoikis resistance, coupled with survival of colorectal tumor …
Introduction TLRs are one of the five major groups of pattern recognition receptors (PRRs) in innate immune cells. Each TLR recognizes distinct microbial components that are shared among groups of microbial species, so-called pathogen-associated molecular …
Abstract Our study aimed to investigate if genetic variants around 16p13.3’s HBA1 locus, associated with erythrocyte indices and HbA1c levels, predict α-thalassemia-related erythrocyte indices, cardiometabolic traits, and diabetes risk in Taiwanese …
Data collection and preprocessing We collected six publicly available scRNA-seq datasets containing cell-type annotations and gene expression values from various scRNA-seq platforms, which can be downloaded from the Gene Expression Omnibus (62) and …
The FML-seq method The protocol for FML-seq comprises only three steps (Fig 1A). First, genomic DNA (gDNA) is digested by a methylation-dependent restriction endonuclease that cuts at a certain distance from the 5-methylcytosine or 5-hydroxymethylcytosine …
Introduction Protein levels are regulated at several nodes. One mode of protein level regulation acts through enhancing or reducing gene transcription. Gene transcription can be stimulated by binding transcription factors to promoters or enhancers of …
Introduction The KCNQ (potassium voltage-gated channel subfamily Q) family of ion channels encode potassium transporters (1). KCNQ proteins typically repolarise the plasma membrane of a cell after depolarisation by allowing the export of potassium ions, …
Introduction The coronavirus infectious diseases-2019 worldwide pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently ongoing after being emerged from Wuhan (China) in December 2019 (Ecdc, 2020; Zhu et al, 2020; …
Introduction Over half of eukaryotic proteins need to be translocated across at least one intracellular membrane to reach the final place of their function. Proteins residing in the mitochondrial matrix are synthesized on cytosolic ribosomes as precursor …
Introduction Themis (or Themis1), is a 72.8-kD protein important in thymocyte development (Fu et al, 2009; Johnson et al, 2009; Kakugawa et al, 2009; Lesourne et al, 2009; Patrick et al, 2009; Ge et al, 2018). It is expressed exclusively in the T cell …
Introduction Gliomas are the most prevalent type of primary brain tumors in adults, and remain among the most difficult cancers to treat (Lapointe et al, 2018). Glioblastoma multiforme (GBM) is a highly malignant World Health Organization (WHO) grade IV …
Abstract Existing monitoring approaches in heart transplantation lack the sensitivity to provide deep molecular assessments to guide management, or require endomyocardial biopsy, an invasive and blind procedure that lacks the precision to reliably obtain …
Abstract SiR-DNA/SiR–Hoechst is a far-red fluorescent DNA probe that is routinely used for live-cell imaging of cell nuclei in interphase and chromosomes during mitosis. Despite being reported to induce DNA damage, SiR-DNA has been used in more than 300 …
Introduction The primary cilium (9 + 0 architecture) is a microtubule-based immotile sensory organelle in vertebrates (Tucker et al, 1979). Enormous progress has been made in the past several decades concerning our understanding of the structure and …
Introduction Single-cell RNA sequencing (RNA-seq) was first achieved by using a quantitative cDNA amplification method and applied to mouse oocytes (Kurimoto et al, 2006; Tang et al, 2009). It has since provided unprecedented opportunities for the study of …
Introduction The retinoblastoma tumor suppressor (RB), which is lost or functionally inactivated in most cancers (Burkhart & Sage, 2008), is best known for its role as a negative regulator of E2F-dependent transcription (Dyson, 1998; Nevins, 2001). As …
Introduction The ongoing severe acute respiratory syndrome coronavirus (SARS-CoV-2) pandemic is associated with millions of deaths and immense pressure on healthcare systems and economies worldwide (1, 2). In most patients, SARS-CoV-2 infection led to a …
