Artistic illustration depicting a head-mounted microscope designed for imaging cerebral oxygenation in a freely behaving mouse.

USA, April 2, 2024 /EINPresswire.com/ -- Scientists developed a new imaging device that enables high-resolution brain imaging in freely moving mice. The featherweight probe, tipping the scale at a mere 4.5 grams, is capable of continuously monitoring cerebral oxygenation and hemodynamic responses with single-vessel resolution.

The brain consumes approximately 25% of the body's oxygen to fuel its neural activities, underscoring the importance of sufficient oxygen supply for maintaining normal cognitive operations. Consequently, monitoring cerebral oxygen levels is pivotal in assessing neurological health, identifying potential brain injuries, and enhancing patient outcomes in critical care settings. Reported in the latest issue of Light Science & Applications, Prof. B. O. Guan and colleagues have introduced an innovative head-mounted microscope. This cutting-edge device enables continuous imaging of oxygenation in mice without restricting their movement.

This new microscope harnesses the capability of label-free photoacoustic imaging for cerebral oxygenation monitoring. It employs an optical fiber to guide and focus laser pulses into biological tissue. An additional fiber optic sensor is incorporated to detect the resulting ultrasound waves triggered by the laser. Taking advantage of the distinct absorption spectra of oxy- and deoxy-genated hemoglobin, the team utilizes dual-colored laser pulses to measure blood oxygen saturation via a spectral unmixing process with single vessel resolution.

This miniature probe can be conveniently affixed to the head of a freely moving mouse, facilitating real-time imaging while observing the oxygenation and hemodynamics of individual blood vessels in the brain.

For instance, the team showcased a short movie created through continuous brain imaging, illustrating the heightened oxygenation in the cortex during the transition from an anesthetized state to wakefulness. Furthermore, they conducted meticulous investigations into the cerebrovascular response to acute hypoxia and hypercapnia challenges, all while the animals were in a freely moving state. This allowed to discern the distinct hemodynamic responses associated with different gas respirations and concentrations. Notably, the imaging device was also utilized to study the impact of obesity on cerebral oxygenation in mice. The imaging findings revealed a significant reduction in the regulatory ability of cerebral vessels to external stimuli in obese mice. Importantly, this head-mounted imager effectively avoids the confounding effects of anesthesia, making it highly favorable and preferable for neuroscience and medical studies.

Cerebral oxygenation is intimately connected to neural activities through the mechanism of neurovascular coupling. Even a brief episode of brain hypoxia can result in irreversible damage and potentially lead to fatal outcomes. Our innovative device opens up new possibilities for detecting brain hypoxia and other injuries, whilst also offering a novel way to study both normal and pathological cerebrovascular functions. Furthermore, it provides invaluable insights into how various behaviors, including motion, influence brain function.

In addition, our imaging probe can be smoothly integrated with existing head-mounted neural imaging techniques, enabling the concurrent investigation of cerebrovascular and neurological activities. This multifaceted device shows promise for studying the neural mechanisms behind social behaviors, as well as neurodegenerative disorders like Alzheimer's disease that disrupt neurovascular couplings. The photoacoustic fiberscope demonstrates immense potential for a broad spectrum of applications, extending beyond neuroscience research.

DOI
10.1038/s41377-023-01348-3

Original Source URL
https://doi.org/10.1038/s41377-023-01348-3

Funding information
This work was supported by National Natural Science Foundation of China under Grant Nos. 62275104, 62322506, 62122031, 62135006 and 61860206002, and the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program under Grant No. 2019BT02X105.

Lucy Wang
BioDesign Research
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